Topo 2 1 plasmid map tutorial

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pET System Manual. 2. Novagen. TB055 8th Edition 02/99. United States & Canada . 1 The pET Peptide Expression System 31 includes host strains BLR and BLR(DE3)pLysS in place of competent cells have a flip top. vector-encoded sequence from the expressed fusion protein upon cleavage with these proteases.
27 Oct 2016 TOPO cloning is a restriction enzyme and ligase free cloning method. topoisomerase I as this enzyme recognizes the DNA sequence 1. Create Your PCR Product: Design standard primers (no need to add unique 2. Set Up TOPO Cloning Reaction: Mix together the PCR product and TOPO Vector. 3.
DES® TOPO® TA Cloning reagents (Box 1) and a box with One Shot® The table below lists the sequence and amount of the primers included in the DES® . pMT/V5-His-TOPO® is a 3.6 kb expression vector designed to facilitate rapid cloning and manual. 2. Produce your PCR product. Page 6, this manual. 3. TOPO®
TOPO® cloning technology allows you to perform one-step benchtop cloning using the new linearized, topoisomerase I-activated pCR™-XL-2-TOPO vector.
26 Feb 2016 Page 2. Gateway and TOPO Cloning Simulation Tutorial. 26 February The sequences and maps of the Invitrogen vectors can be downloaded overhangs at one end of the vector to allow directional cloning of fragments.
pCR®-XL-TOPO®. Parental vector for TOPO® TA cloning of long PCR products. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.
20 Feb 2013
pCR®II-TOPO® (linearized). Linearized vector with 3′-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to
17 Feb 2011
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