Topo cloning invitrogen manual +777+
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27 Oct 2016 Transform TOPO Cloning Reaction into Competent Cells: You can use your standard lab protocol for this; however, you should reduce your
The TOPO TA Cloning® Kits for Sequencing are shipped on dry ice. . Recent experiments at Invitrogen have demonstrated that inclusion of salt (200 mM. NaCl, 10 . plasmid DNA for automated or manual sequencing, we recommend the.
TOPO TA Cloning Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR-TOPO Vector with covalently bound topoisomerase I for fast cloning and Invitrogen™ Manuals & protocols
2 Dec 2013
E-mail: [email protected]. Fax: +31 (0) 50 5299 . TOPO TA Cloning® Kits are available with either pCR®2.1-TOPO® or pCR®II-TOPO® and either
DISCLAIMER. LIFE TECHNOLOGIES CORPORATION AND/OR ITS AFFILIATE(S) DISCLAIM ALL WARRANTIES WITH. RESPECT TO THIS DOCUMENT,
TOPO TA Cloning® Kits are available with either pCR®2.1-TOPO® or . If the purchaser is not willing to accept these use limitations, Invitrogen is willing to .. DNA for automated or manual sequencing, we recommend the S.N.A.P.™
Provides a highly efficient, 5-minute, one-step cloning strategy (“TOPO Cloning”) for the direct insertion of Taq polymerase–amplified PCR products into a24 Feb 2014 TO THE EXTENT ALLOWED BY LAW, LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) WILL . 15 November 2013 • Correction to the TOPO™ Cloning diagram K2800-02, refer to the manual supplied with the miniprep.
27 May 2015
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